A QUANTITATIVE GRAM REACTION

A quantitative gram reaction.

At the present time there is no satisfactory procedure for estimating the degree of gram positivity although the need for a quantitative method is widely recognized. Recently Kennedy and Barbaro (1953) reported an accurate and reproducible method for determining quantitatively the adsorption of crytl violet by bacteria. In addition, the effect of the species and strain of the organism used, the...

A Quantitative Theory of the Precipitin Reaction

1. The reaction between crystalline horse serum albumin and homologous antibody in rabbit sera is quantitatively accounted for by expressions similar to those derived from the law of mass action for other immune precipitating systems. 2. The reaction of an azo dye prepared from crystalline serum albumin by coupling with diazotized R-salt-azo benzidine was also studied with homologous antibody a...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Quantitative Range of Nessler's Reaction with Ammonia.

There is considerable variation in ways of preparing Nessler’s reagent as well as in the manner of forming the colored ammonobasic mercuric iodide. Two critical values are the ratio of KI to Hg19, i.e., x in HgL.xKI, and the final concentration of mercury. The value x determines the stability of solutions and to some extent the relative sensitivity to NH3. Vanselow (2) concluded that x should b...

Evolution of polymerase chain reaction to a quantitative laboratory tool.